Congratulations on the acquisition of NCSA GelReader v 1.0 for the 
Macintosh II!

NCSA GelReader is a product designed to automate to some degree 
the measurement of DNA length using digitized electrophoretic gels. 
First, a digitized gel image is read into the GelReader , and displayed 
as an image. NCSA GelReader has the capability to find the lanes and 
the bands(sites) in the gel. Lanes and bands can be interactivly added 
and deleted if necessary. Then, known molecular weights (standards) 
can be used to approximate the molecular weight of the remaining 
molecules. The result is a text report listing the approximated 
molecular weights of the molecules. Some image enhancement 
filters are also provided.

THIS DISTRIBUTION

Some general information:  All files ending with the extension ".hqx" 
have been encoded by BinHex.  All files with "sit" included in their 
name are archives that have been compressed by StuffIt.

Once you have obtained your files, you will want to extract them.  
For files that have the ".hqx" extension, you will need to use BinHex 
4.0 to extract them.  Run BinHex 4.0 and use the File menu option 
"Upload -> Application".  A dialog will appear asking you for the 
BinHex file you want un-BinHexed.  Once the file has been selected 
another dialog will appear asking where and by what name you want 
to save it as.  (Note:  The new version of Stuffit also has a Decode 
Binhex menu option, which can be used to decode binhex files.)

If the name of the file has an extension ending with ".sit", you will 
want to use UnStuffIt (or Stuffit) to extract the file.  Read the 
StuffIt documentation for details on how to extract the files from 
an archive.

WHAT THIS DISTRIBUTION CONTAINS

Here are the items that you will find in this directory:

1) GRsit.hqx - This is the executable GelReader program.  You will 
need to use BinHex 4.0 to decode GelReader v1.0.  You will then need 
to Unstuff it. Once unstuffed, it can be run.

2) GRsrcsit.hqx - This StuffIt file contains MPW C 3.0 source code 
for GelReader.  You will need to use BinHex 4.0 to decode this file.  
You will then need to Unstuff it.

3) imagesit.hqx - This file contains sample images for GelReader.  
You will need to use BinHex 4.0 to decode this file.  You will then 
need to Unstuff it to extract the images.

4) GRdocsit.hqx - This file contains the documents in Microsoft Word 
format for GelReader.  You will need to use BinHex 4.0 to decode this 
file.  You will then need to Unstuff it to extract the word files.


OBTAINING FREE UPDATES

By purchasing the software the first time, you are automatically 
added to a mailing list. Whenever a more up to date version becomes 
available, you will be notified. You can then get the software free 
from our Anonymous FTP facility. Anonymous FTP is a file server 
mechanism that we provide that will allow you transfer files from 
the NCSA facilities to your home sight. In order to use Anonymous 
FTP, you must have access to the FTP (File Transfer Protocol) 
application and file transfer capability on Internet(ARPANET 
provides for file transfer). If you are not sure that you have these 
capabilities, talk to your network or system administrator.

The Internet address of our anonymous FTP server is 128.174.20.50 
or if your hosts file is up to date then the name zaphod.ncsa.uiuc.edu 
will get you there.

If you have questions about GelReader v 1.0 for the Macintosh II, you 
can contact Jennie File at (217) 244-0638.

Thank you.

Thomas Redman
Project Leader
National Center for Supercomputing Appls.
152 Computing Applications Building
Champaign, IL  61820