                                  showfeat



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   Please help by correcting and extending the Wiki pages.

Function

   Display features of a sequence in pretty format

Description

   showfeat reads one or more protein or nucleic sequences with associated
   feature tables, and writes the features to file (or screen) in a style
   suitable for publication. There are many options for controlling the
   format of the output.

Usage

   Here is a sample session with showfeat


% showfeat
Display features of a sequence in pretty format
Input sequence(s): tembl:x65921
Output file [x65921.showfeat]:


   Go to the input files for this example
   Go to the output files for this example

   Example 2

   Display 'joined' features on one line with positions:


% showfeat -joinfeat -pos
Display features of a sequence in pretty format
Input sequence(s): tembl:x65921
Output file [x65921.showfeat]:


   Go to the output files for this example

   Example 3

   Display just positions and names of CDS features - this can be used as
   a regions file in showseq:


% showfeat -typematch CDS -width 0 -noid -nodesc -noscale -pos
Display features of a sequence in pretty format
Input sequence(s): tembl:x65921
Output file [x65921.showfeat]:


   Go to the output files for this example

Command line arguments

Display features of a sequence in pretty format
Version: EMBOSS:6.5.6.0

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Sequence(s) filename and optional format, or
                                  reference (input USA)
  [-outfile]           outfile    [*.showfeat] Output file name

   Additional (Optional) qualifiers:
   -sourcematch        string     [*] By default any feature source in the
                                  feature table is shown. You can set this to
                                  match any feature source you wish to show.
                                  The source name is usually either the name
                                  of the program that detected the feature or
                                  it is the feature table (eg: EMBL) that the
                                  feature came from.
                                  The source may be wildcarded by using '*'.
                                  If you wish to show more than one source,
                                  separate their names with the character '|',
                                  eg:
                                  gene* | embl (Any string)
   -typematch          string     [*] By default any feature type in the
                                  feature table is shown. You can set this to
                                  match any feature type you wish to show.
                                  See http://www.ebi.ac.uk/embl/WebFeat/ for a
                                  list of the EMBL feature types and see
                                  Appendix A of the Swissprot user manual in
                                  http://www.expasy.org/sprot/userman.html for
                                  a list of the Swissprot feature types.
                                  The type may be wildcarded by using '*'.
                                  If you wish to show more than one type,
                                  separate their names with the character '|',
                                  eg:
                                  *UTR | intron (Any string)
   -tagmatch           string     [*] Tags are the types of extra values that
                                  a feature may have. For example in the EMBL
                                  feature table, a 'CDS' type of feature may
                                  have the tags '/codon', '/codon_start',
                                  '/db_xref', '/EC_number', '/evidence',
                                  '/exception', '/function', '/gene',
                                  '/label', '/map', '/note', '/number',
                                  '/partial', '/product', '/protein_id',
                                  '/pseudo', '/standard_name', '/translation',
                                  '/transl_except', '/transl_table', or
                                  '/usedin'. Some of these tags also have
                                  values, for example '/gene' can have the
                                  value of the gene name.
                                  By default any feature tag in the feature
                                  table is shown. You can set this to match
                                  any feature tag you wish to show.
                                  The tag may be wildcarded by using '*'.
                                  If you wish to show more than one tag,
                                  separate their names with the character '|',
                                  eg:
                                  gene | label (Any string)
   -valuematch         string     [*] Tag values are the values associated
                                  with a feature tag. Tags are the types of
                                  extra values that a feature may have. For
                                  example in the EMBL feature table, a 'CDS'
                                  type of feature may have the tags '/codon',
                                  '/codon_start', '/db_xref', '/EC_number',
                                  '/evidence', '/exception', '/function',
                                  '/gene', '/label', '/map', '/note',
                                  '/number', '/partial', '/product',
                                  '/protein_id', '/pseudo', '/standard_name',
                                  '/translation', '/transl_except',
                                  '/transl_table', or '/usedin'. Only some of
                                  these tags can have values, for example
                                  '/gene' can have the value of the gene name.
                                  By default any feature tag value in the
                                  feature table is shown. You can set this to
                                  match any feature tag value you wish to
                                  show.
                                  The tag value may be wildcarded by using
                                  '*'.
                                  If you wish to show more than one tag value,
                                  separate their names with the character
                                  '|', eg:
                                  pax* | 10 (Any string)
   -sort               menu       [start] Sort features by Type, Start or
                                  Source, Nosort (don't sort - use input
                                  order) or join coding regions together and
                                  leave other features in the input order
                                  (Values: source (Sort by Source); start
                                  (Sort by Start position); type (Sort by
                                  Type); nosort (No sorting done))
   -joinfeatures       boolean    [N] Join coding regions together
   -annotation         range      [If this is left blank, then no annotation
                                  is added.] Regions to annotate by marking.
                                  If this is left blank, then no annotation is
                                  added.
                                  A set of regions is specified by a set of
                                  pairs of positions followed by optional
                                  text.
                                  The positions are integers.
                                  They are followed by any text (but not
                                  digits when on the command-line).
                                  Examples of region specifications are:
                                  24-45 new domain 56-78 match to Mouse
                                  1-100 First part 120-156 oligo
                                  A file of ranges to annotate (one range per
                                  line) can be specified as '@filename'.

   Advanced (Unprompted) qualifiers:
   -html               boolean    [N] Use HTML formatting
   -[no]id             boolean    [Y] Set this to be false if you do not wish
                                  to display the ID name of the sequence.
   -[no]description    boolean    [Y] Set this to be false if you do not wish
                                  to display the description of the sequence.
   -[no]scale          boolean    [Y] Set this to be false if you do not wish
                                  to display the scale line.
   -width              integer    [60] You can expand (or contract) the width
                                  of the ASCII-character graphics display of
                                  the positions of the features using this
                                  value.
                                  For example, a width of 80 characters would
                                  cover a standard page width and a width a 10
                                  characters would be nearly unreadable.
                                  If the width is set to less than 4, the
                                  graphics lines and the scale line will not
                                  be displayed. (Integer 0 or more)
   -collapse           boolean    [N] If this is set, then features from the
                                  same source and of the same type and sense
                                  are all printed on the same line. For
                                  instance if there are several features from
                                  the EMBL feature table (ie. the same source)
                                  which are all of type 'exon' in the same
                                  sense, then they will all be displayed on
                                  the same line. This makes it hard to
                                  distinguish overlapping features.
                                  If this is set to false then each feature is
                                  displayed on a separate line making it
                                  easier to distinguish where features start
                                  and end.
   -[no]forward        boolean    [Y] Set this to be false if you do not wish
                                  to display forward sense features.
   -[no]reverse        boolean    [Y] Set this to be false if you do not wish
                                  to display reverse sense features.
   -[no]unknown        boolean    [Y] Set this to be false if you do not wish
                                  to display unknown sense features. (ie.
                                  features with no directionality - all
                                  protein features are of this type and some
                                  nucleic features (for example, CG-rich
                                  regions)).
   -strand             boolean    [N] Set this if you wish to display the
                                  strand of the features. Protein features are
                                  always directionless (indicated by '0'),
                                  forward is indicated by '+' and reverse is
                                  '-'.
   -sourceshow         boolean    [N] Set this if you wish to display the
                                  origin of the features.
                                  The source name is usually either the name
                                  of the program that detected the feature or
                                  it is the name of the feature table (eg:
                                  EMBL) that the feature came from.
   -position           boolean    [N] Set this if you wish to display the
                                  start and end position of the features. If
                                  several features are being displayed on the
                                  same line, then the start and end positions
                                  will be joined by a comma, for example:
                                  '189-189,225-225'.
   -[no]typeshow       boolean    [Y] Set this to be false if you do not wish
                                  to display the type of the features.
   -tagshow            boolean    [N] Set this to be false if you do not wish
                                  to display the tags and values of the
                                  features.
   -[no]valueshow      boolean    [Y] Set this to be false if you do not wish
                                  to display the tag values of the features.
                                  If this is set to be false, only the tag
                                  names will be displayed. If the tags are not
                                  displayed, then the values will not be
                                  displayed. The value of the 'translation'
                                  tag is never displayed as it is often
                                  extremely long.
   -stricttags         boolean    [N] By default if any tag/value pair in a
                                  feature matches the specified tag and value,
                                  then all the tags/value pairs of that
                                  feature will be displayed. If this is set to
                                  be true, then only those tag/value pairs in
                                  a feature that match the specified tag and
                                  value will be displayed.

   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -scircular1         boolean    Sequence is circular
   -sformat1           string     Input sequence format
   -iquery1            string     Input query fields or ID list
   -ioffset1           integer    Input start position offset
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -odirectory2        string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit


Input file format

   The input is a standard EMBOSS sequence query (also known as a 'USA')
   with associated feature information.

   Major sequence database sources defined as standard in EMBOSS
   installations include srs:embl, srs:uniprot and ensembl

   Data can also be read from sequence output in any supported format
   written by an EMBOSS or third-party application.

   The input format can be specified by using the command-line qualifier
   -sformat xxx, where 'xxx' is replaced by the name of the required
   format. The available format names are: text, html, xml (uniprotxml),
   obo, embl (swissprot)

   Where the sequence format has no feature information, a second file can
   be read to load the feature data. The file is specified with the
   qualifier -ufo xxx and the feature format is specified with the
   qualifier -fformat xxx

   See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further
   information on sequence formats.

   See: http://emboss.sf.net/docs/themes/FeatureFormats.html for further
   information on feature formats.

  Input files for usage example

   'tembl:x65921' is a sequence entry in the example nucleic acid database
   'tembl'

  Database entry: tembl:x65921

ID   X65921; SV 1; linear; genomic DNA; STD; HUM; 2016 BP.
XX
AC   X65921; S45242;
XX
DT   13-MAY-1992 (Rel. 31, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 7)
XX
DE   H.sapiens fau 1 gene
XX
KW   fau 1 gene.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-2016
RA   Kas K.;
RT   ;
RL   Submitted (29-APR-1992) to the INSDC.
RL   K. Kas, University of Antwerp, Dept of Biochemistry T3.22,
RL   Universiteitsplein 1, 2610 Wilrijk, BELGIUM
XX
RN   [2]
RP   1-2016
RX   DOI; 10.1016/0006-291X(92)91286-Y.
RX   PUBMED; 1326960.
RA   Kas K., Michiels L., Merregaert J.;
RT   "Genomic structure and expression of the human fau gene: encoding the
RT   ribosomal protein S30 fused to a ubiquitin-like protein";
RL   Biochem. Biophys. Res. Commun. 187(2):927-933(1992).
XX
DR   Ensembl-Gn; ENSG00000149806; Homo_sapiens.
DR   Ensembl-Tr; ENST00000279259; Homo_sapiens.
DR   Ensembl-Tr; ENST00000434372; Homo_sapiens.
DR   Ensembl-Tr; ENST00000525297; Homo_sapiens.
DR   Ensembl-Tr; ENST00000526555; Homo_sapiens.
DR   Ensembl-Tr; ENST00000527548; Homo_sapiens.
DR   Ensembl-Tr; ENST00000529259; Homo_sapiens.
DR   Ensembl-Tr; ENST00000529639; Homo_sapiens.
DR   Ensembl-Tr; ENST00000531743; Homo_sapiens.
DR   GDB; 191789.
DR   GDB; 191790.
DR   GDB; 354872.
DR   GDB; 4590236.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2016


  [Part of this file has been deleted for brevity]

FT                   RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
FT   intron          857..950
FT                   /number=2
FT   exon            951..1095
FT                   /number=3
FT   intron          1096..1556
FT                   /number=3
FT   exon            1557..1612
FT                   /number=4
FT   intron          1613..1786
FT                   /number=4
FT   exon            1787..>1912
FT                   /number=5
FT   polyA_signal    1938..1943
XX
SQ   Sequence 2016 BP; 421 A; 562 C; 538 G; 495 T; 0 other;
     ctaccatttt ccctctcgat tctatatgta cactcgggac aagttctcct gatcgaaaac        60
     ggcaaaacta aggccccaag taggaatgcc ttagttttcg gggttaacaa tgattaacac       120
     tgagcctcac acccacgcga tgccctcagc tcctcgctca gcgctctcac caacagccgt       180
     agcccgcagc cccgctggac accggttctc catccccgca gcgtagcccg gaacatggta       240
     gctgccatct ttacctgcta cgccagcctt ctgtgcgcgc aactgtctgg tcccgccccg       300
     tcctgcgcga gctgctgccc aggcaggttc gccggtgcga gcgtaaaggg gcggagctag       360
     gactgccttg ggcggtacaa atagcaggga accgcgcggt cgctcagcag tgacgtgaca       420
     cgcagcccac ggtctgtact gacgcgccct cgcttcttcc tctttctcga ctccatcttc       480
     gcggtagctg ggaccgccgt tcaggtaaga atggggcctt ggctggatcc gaagggcttg       540
     tagcaggttg gctgcggggt cagaaggcgc ggggggaacc gaagaacggg gcctgctccg       600
     tggccctgct ccagtcccta tccgaactcc ttgggaggca ctggccttcc gcacgtgagc       660
     cgccgcgacc accatcccgt cgcgatcgtt tctggaccgc tttccactcc caaatctcct       720
     ttatcccaga gcatttcttg gcttctctta caagccgtct tttctttact cagtcgccaa       780
     tatgcagctc tttgtccgcg cccaggagct acacaccttc gaggtgaccg gccaggaaac       840
     ggtcgcccag atcaaggtaa ggctgcttgg tgcgccctgg gttccatttt cttgtgctct       900
     tcactctcgc ggcccgaggg aacgcttacg agccttatct ttccctgtag gctcatgtag       960
     cctcactgga gggcattgcc ccggaagatc aagtcgtgct cctggcaggc gcgcccctgg      1020
     aggatgaggc cactctgggc cagtgcgggg tggaggccct gactaccctg gaagtagcag      1080
     gccgcatgct tggaggtgag tgagagagga atgttctttg aagtaccggt aagcgtctag      1140
     tgagtgtggg gtgcatagtc ctgacagctg agtgtcacac ctatggtaat agagtacttc      1200
     tcactgtctt cagttcagag tgattcttcc tgtttacatc cctcatgttg aacacagacg      1260
     tccatgggag actgagccag agtgtagttg tatttcagtc acatcacgag atcctagtct      1320
     ggttatcagc ttccacacta aaaattaggt cagaccaggc cccaaagtgc tctataaatt      1380
     agaagctgga agatcctgaa atgaaactta agatttcaag gtcaaatatc tgcaactttg      1440
     ttctcattac ctattgggcg cagcttctct ttaaaggctt gaattgagaa aagaggggtt      1500
     ctgctgggtg gcaccttctt gctcttacct gctggtgcct tcctttccca ctacaggtaa      1560
     agtccatggt tccctggccc gtgctggaaa agtgagaggt cagactccta aggtgagtga      1620
     gagtattagt ggtcatggtg ttaggacttt ttttcctttc acagctaaac caagtccctg      1680
     ggctcttact cggtttgcct tctccctccc tggagatgag cctgagggaa gggatgctag      1740
     gtgtggaaga caggaaccag ggcctgatta accttccctt ctccaggtgg ccaaacagga      1800
     gaagaagaag aagaagacag gtcgggctaa gcggcggatg cagtacaacc ggcgctttgt      1860
     caacgttgtg cccacctttg gcaagaagaa gggccccaat gccaactctt aagtcttttg      1920
     taattctggc tttctctaat aaaaaagcca cttagttcag tcatcgcatt gtttcatctt      1980
     tacttgcaag gcctcaggga gaggtgtgct tctcgg                                2016
//

Output file format

   The output is a text representation of the feature table.

  Output files for usage example

  File: x65921.showfeat

X65921
H.sapiens fau 1 gene
|==========================================================| 2016
|----------------------------------------------------------> source
           |-->                                              exon
           |-->                                              mRNA
                      |->                                    mRNA
                           |--->                             mRNA
                                             |>              mRNA
                                                    |-->     mRNA
              |------->                                      intron
                      |->                                    exon
                      |->                                    CDS
                           |--->                             CDS
                                             |>              CDS
                                                    |-->     CDS
                        |-->                                 intron
                           |--->                             exon
                               |------------->               intron
                                             |>              exon
                                               |---->        intron
                                                    |-->     exon
                                                        >    polyA_signal


  Output files for usage example 2

  File: x65921.showfeat

X65921
H.sapiens fau 1 gene
|==========================================================| 2016
|----------------------------------------------------------> 1-2016 source
           |-->                                              408-504 exon
           |-->       |->  |--->             |>     |-->     408-504,774-856,951
-1095,1557-1612,1787-1912 mRNA
              |------->                                      505-773 intron
                      |->                                    774-856 exon
                      |->  |--->             |>     |-->     782-856,951-1095,15
57-1612,1787-1912 CDS
                        |-->                                 857-950 intron
                           |--->                             951-1095 exon
                               |------------->               1096-1556 intron
                                             |>              1557-1612 exon
                                               |---->        1613-1786 intron
                                                    |-->     1787-1912 exon
                                                        >    1938-1943 polyA_sig
nal


  Output files for usage example 3

  File: x65921.showfeat

782-856 CDS
951-1095 CDS
1557-1612 CDS
1787-1912 CDS


Data files

   None.

Notes

   Sequence regions to annotate may be specified as a set or ranges.
   Optionally, forward and reverse sense features, and features of
   unspecified sense (for example, CG-rich regions), are displayed.
   Optionally, features from the same source and of the same type and
   sense may all be printed on the same line, although this may make it
   hard to distinguish overlapping features.

   By default, any features in the feature table are displayed however
   there are options to restrict this by feature source, type, feature tag
   and feature tag value.

   Features may be sorted by Type, Start or Source, left unsorted (in
   which case the same order as the input is used), or features for the
   coding regions presented all together.

   Various options control what else is displayed, including the sequence
   ID, description, scale line, width of display, strand of the features,
   feature source, start and end positions, feature type, and feature tags
   and tag values. By default if any tag/value pair in a feature matches
   the specified tag and value, then all the tags/value pairs of that
   feature will be displayed. If this is set to be true, then only those
   tag/value pairs in a feature that match the specified tag and value
   will be displayed.

   Optionally, output can be in HTML.

References

   None.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with a status of 0.

Known bugs

   None.

See also

                   Program name                            Description
                   abiview      Display the trace in an ABI sequencer file
                   cirdna       Draw circular map of DNA constructs
                   extractfeat  Extract features from sequence(s)
                   iep          Calculate the isoelectric point of proteins
                   lindna       Draw linear maps of DNA constructs
                   maskfeat     Write a sequence with masked features
                   pepinfo      Plot amino acid properties of a protein sequence in parallel
                   pepnet       Draw a helical net for a protein sequence
                   pepwheel     Draw a helical wheel diagram for a protein sequence
                   plotorf      Plot potential open reading frames in a nucleotide sequence
                   prettyplot   Draw a sequence alignment with pretty formatting
                   prettyseq    Write a nucleotide sequence and its translation to file
                   remap        Display restriction enzyme binding sites in a nucleotide sequence
                   showpep      Display protein sequences with features in pretty format
                   sixpack      Display a DNA sequence with 6-frame translation and ORFs
                   twofeat      Find neighbouring pairs of features in sequence(s)

Author(s)

   Gary             Williams formerly at:
   MRC              Rosalind Franklin Centre for Genomics Research Wellcome Trust
   Genome           Campus, Hinxton, Cambridge, CB10 1SB, UK

                    Please report all bugs to the EMBOSS bug team
                    (emboss-bug (c) emboss.open-bio.org) not to the original author.

History

                    Written 1999 - Gary Williams

                    Dec 2001 - added -sort nosort option to get the features in the input
                    order

Target users

                    This program is intended to be used by everyone and everything, from
                    naive users to embedded scripts.

Comments

                    None
