EMBOSS: complex

| complex

## Wiki

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Please help by correcting and extending the Wiki pages.

## Function

Find the linguistic complexity in nucleotide sequences

## Description

## Usage

Here is a sample session with complex

    % complex -omnia
    Find the linguistic complexity in nucleotide sequences
    Input nucleotide sequence(s): tembl:x*
    Window length [100]:
    Step size [5]:
    Minimum word length [4]:
    Maximum word length [6]:
    Program complex output file [x59796.complex]:
    output sequence(s) [x59796.fasta]:

Go to the input files for this example
Go to the output files for this example

## Command line arguments

    Find the linguistic complexity in nucleotide sequences
    Version: EMBOSS:6.3.1

       Standard (Mandatory) qualifiers (* if not always prompted):
      [-sequence]          seqall     Nucleotide sequence(s) filename and optional
                                      format, or reference (input USA)
       -lwin               integer    [100] Window length (Integer 1 or more)
       -step               integer    [5] Displacement of the window over the
                                      sequence (Integer 1 or more)
       -jmin               integer    [4] Minimum word length (Integer from 2 to
                                      20)
       -jmax               integer    [6] Maximum word length (Integer from 2 to
                                      50)
      [-outfile]           outfile    [*.complex] Program complex output file
    *  -outseq             seqoutall  [.] Sequence set(s)
                                      filename and optional format (output USA)

       Additional (Optional) qualifiers: (none)
       Advanced (Unprompted) qualifiers:
       -omnia              toggle     [N] Calculate over a set of sequences
       -sim                integer    [0] Calculate the linguistic complexity by
                                      comparison with a number of simulations
                                      having a uniform distribution of bases (Any
                                      integer value)
       -freq               boolean    [N] Execute the simulation of a sequence
                                      based on the base frequency of the original
                                      sequence
       -print              boolean    [N] Generate a file named UjTable containing
                                      the values of Uj for each word j in the
                                      real sequence(s) and in any simulated
                                      sequences
       -ujtablefile        outfile    [complex.ujtable] Program complex temporary
                                      output file

       Associated qualifiers:

       "-sequence" associated qualifiers
       -sbegin1            integer    Start of each sequence to be used
       -send1              integer    End of each sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -sformat1           string     Input sequence format
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-outfile" associated qualifiers
       -odirectory2        string     Output directory

       "-ujtablefile" associated qualifiers
       -odirectory         string     Output directory

       "-outseq" associated qualifiers
       -osformat           string     Output seq format
       -osextension        string     File name extension
       -osname             string     Base file name
       -osdirectory        string     Output directory
       -osdbname           string     Database name to add
       -ossingle           boolean    Separate file for each entry
       -oufo               string     UFO features
       -offormat           string     Features format
       -ofname             string     Features file name
       -ofdirectory        string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-sequence] (Parameter 1) | seqall | Nucleotide sequence(s) filename and optional format, or reference (input USA) | Readable sequence(s) | Required
-lwin | integer | Window length | Integer 1 or more | 100
-step | integer | Displacement of the window over the sequence | Integer 1 or more | 5
-jmin | integer | Minimum word length | Integer from 2 to 20 | 4
-jmax | integer | Maximum word length | Integer from 2 to 50 | 6
[-outfile] (Parameter 2) | outfile | Program complex output file | Output file | <*>.complex
-outseq | seqoutall | Sequence set(s) filename and optional format (output USA) | Writeable sequence(s) | <*>.format
Additional (Optional) qualifiers
(none)
Advanced (Unprompted) qualifiers
-omnia | toggle | Calculate over a set of sequences | Toggle value Yes/No | No
-sim | integer | Calculate the linguistic complexity by comparison with a number of simulations having a uniform distribution of bases | Any integer value | 0
-freq | boolean | Execute the simulation of a sequence based on the base frequency of the original sequence | Boolean value Yes/No | No
-print | boolean | Generate a file named UjTable containing the values of Uj for each word j in the real sequence(s) and in any simulated sequences | Boolean value Yes/No | No
-ujtablefile | outfile | Program complex temporary output file | Output file | complex.ujtable
Associated qualifiers
"-sequence" associated seqall qualifiers
-sbegin1 -sbegin_sequence | integer | Start of each sequence to be used | Any integer value | 0
-send1 -send_sequence | integer | End of each sequence to be used | Any integer value | 0
-sreverse1 -sreverse_sequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_sequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_sequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_sequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_sequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_sequence | boolean | Make upper case | Boolean value Yes/No | N
-sformat1 -sformat_sequence | string | Input sequence format | Any string |
-sdbname1 -sdbname_sequence | string | Database name | Any string |
-sid1 -sid_sequence | string | Entryname | Any string |
-ufo1 -ufo_sequence | string | UFO features | Any string |
-fformat1 -fformat_sequence | string | Features format | Any string |
-fopenfile1 -fopenfile_sequence | string | Features file name | Any string |
"-outfile" associated outfile qualifiers
-odirectory2 -odirectory_outfile | string | Output directory | Any string |
"-ujtablefile" associated outfile qualifiers
-odirectory | string | Output directory | Any string |
"-outseq" associated seqoutall qualifiers
-osformat | string | Output seq format | Any string |
-osextension | string | File name extension | Any string |
-osname | string | Base file name | Any string |
-osdirectory | string | Output directory | Any string |
-osdbname | string | Database name to add | Any string |
-ossingle | boolean | Separate file for each entry | Boolean value Yes/No | N
-oufo | string | UFO features | Any string |
-offormat | string | Features format | Any string |
-ofname | string | Features file name | Any string |
-ofdirectory | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

### Input files for usage example

'tembl:x*' is a sequence entry in the example nucleic acid database 'tembl'

## Output file format

Sequence TEMBL:HHTETRA contains repeats and is included in the test database for repeat analysis.

### Output files for usage example

### File: complex.ujtable

### File: x59796.complex

    Length of window : 100
    jmin : 4
    jmax : 6
    step : 5
    Execution without simulation
    ----------------------------------------------------------------------------
    |                  |                  |                  |                  |
    |     number of    |      name of     |     length of    |      value of    |
    |     sequence     |     sequence     |     sequence     |     complexity   |
    |                  |                  |                  |                  |
    ----------------------------------------------------------------------------
             1                      X59796           3170             0.6921
             2                      X65923            518             0.6739
             3                      X65921           2016             0.7105
             4                      X51466           3075             0.6925
             5                      X07523           1658             0.7314
             6                      X03487            512             0.5609
             7                      X03488           1132             0.7217
             8                      X07797           1675             0.6201
             9                      X51872           1832             0.6916
            10                      X77160           1212             0.6596
            11                      X13776           2167             0.6562
            12                      X77161           1130             0.6989

### File: x59796.fasta

    >X59796 X59796.1 H.sapiens mRNA for cadherin-5
    ctccactcacgctcagccctggacggacaggcagtccaacggaacagaaacatccctcag
    cccacaggcacgatctgttcctcctgggaagatgcagaggctcatgatgctcctcgccac
    atcgggcgcctgcctgggcctgctggcagtggcagcagtggcagcagcaggtgctaaccc
    tgcccaacgggacacccacagcctgctgcccacccaccggcgccaaaagagagattggat
    ttggaaccagatgcacattgatgaagagaaaaacacctcacttccccatcatgtaggcaa
    gatcaagtcaagcgtgagtcgcaagaatgccaagtacctgctcaaaggagaatatgtggg
    caaggtcttccgggtcgatgcagagacaggagacgtgttcgccattgagaggctggaccg
    ggagaatatctcagagtaccacctcactgctgtcattgtggacaaggacactggcgaaaa
    cctggagactccttccagcttcaccatcaaagttcatgacgtgaacgacaactggcctgt
    gttcacgcatcggttgttcaatgcgtccgtgcctgagtcgtcggctgtggggacctcagt
    catctctgtgacagcagtggatgcagacgaccccactgtgggagaccacgcctctgtcat
    gtaccaaatcctgaaggggaaagagtattttgccatcgataattctggacgtattatcac
    aataacgaaaagcttggaccgagagaagcaggccaggtatgagatcgtggtggaagcgcg
    agatgcccagggcctccggggggactcgggcacggccaccgtgctggtcactctgcaaga
    catcaatgacaacttccccttcttcacccagaccaagtacacatttgtcgtgcctgaaga
    cacccgtgtgggcacctctgtgggctctctgtttgttgaggacccagatgagccccagaa
    ccggatgaccaagtacagcatcttgcggggcgactaccaggacgctttcaccattgagac
    aaaccccgcccacaacgagggcatcatcaagcccatgaagcctctggattatgaatacat
    ccagcaatacagcttcatagtcgaggccacagaccccaccatcgacctccgatacatgag
    ccctcccgcgggaaacagagcccaggtcattatcaacatcacagatgtggacgagccccc
    cattttccagcagcctttctaccacttccagctgaaggaaaaccagaagaagcctctgat
    tggcacagtgctggccatggaccctgatgcggctaggcatagcattggatactccatccg
    caggaccagtgacaagggccagttcttccgagtcacaaaaaagggggacatttacaatga
    gaaagaactggacagagaagtctacccctggtataacctgactgtggaggccaaagaact
    ggattccactggaacccccacaggaaaagaatccattgtgcaagtccacattgaagtttt
    ggatgagaatgacaatgccccggagtttgccaagccctaccagcccaaagtgtgtgagaa
    cgctgtccatggccagctggtcctgcagatctccgcaatagacaaggacataacaccacg
    aaacgtgaagttcaaattcatcttgaatactgagaacaactttaccctcacggataatca
    cgataacacggccaacatcacagtcaagtatgggcagtttgaccgggagcataccaaggt
    ccacttcctacccgtggtcatctcagacaatgggatgccaagtcgcacgggcaccagcac
    gctgaccgtggccgtgtgcaagtgcaacgagcagggcgagttcaccttctgcgaggatat
    ggccgcccaggtgggcgtgagcatccaggcagtggtagccatcttactctgcatcctcac
    catcacagtgatcaccctgctcatcttcctgcggcggcggctccggaagcaggcccgcgc
    gcacggcaagagcgtgccggagatccacgagcagctggtcacctacgacgaggagggcgg
    cggcgagatggacaccaccagctacgatgtgtcggtgctcaactcggtgcgccgcggcgg
    ggccaagcccccgcggcccgcgctggacgcccggccttccctctatgcgcaggtgcagaa
    gccaccgaggcacgcgcctggggcacacggagggcccggggagatggcagccatgatcga
    ggtgaagaaggacgaggcggaccacgacggcgacggccccccctacgacacgctgcacat
    ctacggctacgagggctccgagtccatagccgagtccctcagctccctgggcaccgactc
    atccgactctgacgtggattacgacttccttaacgactggggacccaggtttaagatgct
    ggctgagctgtacggctcggacccccgggaggagctgctgtattaggcggccgaggtcac
    tctgggcctggggacccaaaccccctgcagcccaggccagtcagactccaggcaccacag
    cvncadctccaaaaatggcagtgactccccagcccagcaccccttcctcgtgggtcccag
    agacctcatcagccttgggatagcaaactccaggttcctgaaatatccaggaatatatgt
    cagtgatgactattctcaaatgctggcaaatccaggctggtgttctgtctgggctcagac
    atccacataaccctgtcacccacagaccgccgtctaactcaaagacttcctctggctccc
    caaggctgcaaagcaaaacagactgtgtttaactgctgcagggtctttttctagggtccc
    tgaacgccctggtaaggctggtgaggtcctggtgcctatctgcctggaggcaaaggcctg
    gacagcttgacttgtggggcaggattctctgcagcccattcccaagggagactgaccatc

      [Part of this file has been deleted for brevity]

    tacggttcctcgtgggctgctacatgtcgcacacgcgcaaggcggtgatgccggtggtcg
    agcgcgccgacgcgctgctctgctacccgaccccctacgagggcttcgagtattcgccga
    acatcgtctacggcggtccggcgccgaaccagaacagtgcgccgctggcggcgtacctga
    ttcgccactacggcgagcgggtggtgttcatcggctcggactacatctatccgcgggaaa
    gcaaccatgtgatgcgccacctgtatcgccagcacggcggcacggtgctcgaggaaatct
    acattccgctgtatccctccgacgacgacttgcagcgcgccgtcgagcgcatctaccagg
    cgcgcgccgacgtggtcttctccaccgtggtgggcaccggcaccgccgagctgtatcgcg
    ccatcgcccgtcgctacggcgacggcaggcggccgccgatcgccagcctgaccaccagcg
    aggcggaggtggcgaagatggagagtgacgtggcagaggggcaggtggtggtcgcgcctt
    acttctccagcatcgatacgcccgccagccgggccttcgtccaggcctgccatggtttct
    tcccggagaacgcgaccatcaccgcctgggccgaggcggcctactggcagaccttgttgc
    tcggccgcgccgcgcaggccgcaggcaactggcgggtggaagacgtgcagcggcacctgt
    acgacatcgacatcgacgcgccacaggggccggtccgggtggagcgccagaacaaccaca
    gccgcctgtcttcgcgcatcgcggaaatcgatgcgcgcggcgtgttccaggtccgctggc
    agtcgcccgaaccgattcgccccgacccttatgtcgtcgtgcataacctcgacgactggt
    ccgccagcatgggcgggggaccgctcccatgagcgccaactcgctgctcggcagcctgcg
    cgagttgcaggtgctggtcctcaacccgccgggggaggtcagcgacgccctggtcttgca
    gctgatccgcatcggttgttcggtgcgccagtgctggccgccgccggaagccttcgacgt
    gccggtggacgtggtcttcaccagcattttccagaatggccaccacgacgagatcgctgc
    gctgctcgccgccgggactccgcgcactaccctggtggcgctggtggagtacgaaagccc
    cgcggtgctctcgcagatcatcgagctggagtgccacggcgtgatcacccagccgctcga
    tgcccaccgggtgctgcctgtgctggtatcggcgcggcgcatcagcgaggaaatggcgaa
    gctgaagcagaagaccgagcagctccaggaccgcatcgccggccaggcccggatcaacca
    ggccaaggtgttgctgatgcagcgccatggctgggacgagcgcgaggcgcaccagcacct
    gtcgcgggaagcgatgaagcggcgcgagccgatcctgaagatcgctcaggagttgctggg
    aaacgagccgtccgcctgagcgatccgggccgaccagaacaataacaagaggggtatcgt
    catcatgctgggactggttctgctgtacgttggcgcggtgctgtttctcaatgccgtctg
    gttgctgggcaagatcagcggtcgggaggtggcggtgatcaacttcctggtcggcgtgct
    gagcgcctgcgtcgcgttctacctgatcttttccgcagcagccgggcagggctcgctgaa
    ggccggagcgctgaccctgctattcgcttttacctatctgtgggtggccgccaaccagtt
    cctcgag
    >X77161 X77161.1 Pseudomonas aeruginosa (PAC1) amiS gene.
    gagccgtccgcctgagcgatccgggccgaccagaacaataacaagaggggtatcgtcatc
    atgctgggactggttctgctgtacgttggcgcggtgctgtttctcaatgccgtctggttg
    ctgggcaagatcagcggtcgggaggtggcggtgatcaacttcctggtcggcgtgctgagc
    gcctgcgtcgcgttctacctgatcttttccgcagcagccgggcagggctcgctgaaggcc
    ggagcgctgaccctgctattcgcttttacctatctgtgggtggccgccaaccagttcctc
    gaggtggacggcaagggcctcggctggttctgcctgttcgtcagcctcaccgcctgcacc
    gtggcgatcgagtcgttcgccggcgccagtggtccgttcggcctgtggaacgcggtcaac
    tggacagtctgggcgttgctctggttctgtttcttcctgctgctggggctgtcccgcggc
    atccagaagccggtggcctacctgaccctggccagcgccatattcaccgcctggttgccc
    ggcctgctgctgctcggacaggtgctcaaggcatagcaggaagtcggaaagggatgacgg
    cttgccgccatcccgtcccttccgaacgcctagccgagcggccagttgatcaccacgacg
    gcgtcgttgtagtcgttgtcggtgccgtcttcagagccgaccagggcgaagttcagctcg
    ttggtcaggattacctgtgccgagaccagatccgaggggcggccgttgacgctgacctgg
    acctgtaccttgccactgctgccggagttgagcacctgggtgccgatgacggcgttattg
    gtgctttgcccgctgaaggtcgcggccgtgctcgttgttgaccagcacgttcaccgtctg
    ggttccggacgagttggcgaaggcggtgacgccggaacctggttgttggcgggaagggtg
    aacactccttgtggttgccatggtggtatctccactgaatacctggccccttccttttca
    ggcagccgtctggcgcgcggtatggcgtgtcgggagaaatccgcagtccttggcggcagg
    cgatgcgcaggcaggaaggacgcatcgttcagccaatctacgccgtcgac

## Data files

None

## Notes

None

## References

None

## Warnings

None

## Diagnostic Error Messages

None

## Exit status

Always exits with status 0

## Known bugs

None

## See also

Program name | Description
--- | ---
banana | Plot bending and curvature data for B-DNA
btwisted | Calculate the twisting in a B-DNA sequence
chaos | Draw a chaos game representation plot for a nucleotide sequence
compseq | Calculate the composition of unique words in sequences
dan | Calculates nucleic acid melting temperature
density | Draw a nucleic acid density plot
freak | Generate residue/base frequency table or plot
isochore | Plots isochores in DNA sequences
sirna | Finds siRNA duplexes in mRNA
wordcount | Count and extract unique words in molecular sequence(s)

## Author(s)

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None