 
REBASE version 402                                              proto.402
 
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    REBASE, The Restriction Enzyme Database   http://rebase.neb.com
    Copyright (c)  Dr. Richard J. Roberts, 2014.   All rights reserved.
    =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
 
Rich Roberts                                                    Jan 30 2014
 



	    TYPE II ENZYMES
	    ---------------

AarI                           CACCTGC (4/8)
AatII                          GACGT^C
AbaCIII                        CTATCAV
AbsI                           CC^TCGAGG
AccI                           GT^MKAC
AceIII                         CAGCTC (7/11)
AciI                           CCGC (-3/-1)
AclI                           AA^CGTT
AcyI                           GR^CGYC
AflII                          C^TTAAG
AflIII                         A^CRYGT
AgeI                           A^CCGGT
AgsI                           TTS^AA
AhaIII                         TTT^AAA
AjuI                           (7/12) GAANNNNNNNTTGG (11/6)
AlfI                           (10/12) GCANNNNNNTGC (12/10)
AloI                           (7/12) GAACNNNNNNTCC (12/7)
AluI                           AG^CT
AlwFI                          GAAAYNNNNNRTG
AlwNI                          CAGNNN^CTG
AoxI                           ^GGCC
ApaI                           GGGCC^C
ApaBI                          GCANNNNN^TGC
ApaLI                          G^TGCAC
ApoI                           R^AATTY
ApyPI                          ATCGAC (20/18)
AquII                          GCCGNAC (20/18)
AquIV                          GRGGAAG (19/17)
ArsI                           (8/13) GACNNNNNNTTYG (11/6)
AscI                           GG^CGCGCC
AspBHI                         YSCNS (8/12)
AsuI                           G^GNCC
AsuII                          TT^CGAA
AvaI                           C^YCGRG
AvaII                          G^GWCC
AvaIII                         ATGCAT
AvrII                          C^CTAGG
BaeI                           (10/15) ACNNNNGTAYC (12/7)
BalI                           TGG^CCA
BamHI                          G^GATCC
BarI                           (7/12) GAAGNNNNNNTAC (12/7)
BbvI                           GCAGC (8/12)
BbvII                          GAAGAC (2/6)
BbvCI                          CCTCAGC (-5/-2)
BccI                           CCATC (4/5)
Bce83I                         CTTGAG (16/14)
BcefI                          ACGGC (12/13)
BcgI                           (10/12) CGANNNNNNTGC (12/10)
BciVI                          GTATCC (6/5)
BclI                           T^GATCA
BdaI                           (10/12) TGANNNNNNTCA (12/10)
BetI                           W^CCGGW
BfiI                           ACTGGG (5/4)
BglI                           GCCNNNN^NGGC
BglII                          A^GATCT
BinI                           GGATC (4/5)
BisI                           GC^NGC
BmgI                           GKGCCC
BplI                           (8/13) GAGNNNNNCTC (13/8)
Bpu10I                         CCTNAGC (-5/-2)
BsaAI                          YAC^GTR
BsaBI                          GATNN^NNATC
BsaXI                          (9/12) ACNNNNNCTCC (10/7)
BsbI                           CAACAC (21/19)
BscGI                          CCCGT
BseMII                         CTCAG (10/8)
BsePI                          G^CGCGC
BseRI                          GAGGAG (10/8)
BseSI                          GKGCM^C
BseYI                          CCCAGC (-5/-1)
BsgI                           GTGCAG (16/14)
BsiI                           CACGAG (-5/-1)
BsiYI                          CCNNNNN^NNGG
BsmI                           GAATGC (1/-1)
BsmAI                          GTCTC (1/5)
Bsp24I                         (8/13) GACNNNNNNTGG (12/7)
Bsp1407I                       T^GTACA
BspGI                          CTGGAC
BspHI                          T^CATGA
BspLU11I                       A^CATGT
BspMI                          ACCTGC (4/8)
BspMII                         T^CCGGA
BspNCI                         CCAGA
BsrI                           ACTGG (1/-1)
BsrBI                          CCGCTC (-3/-3)
BsrDI                          GCAATG (2/0)
BstEII                         G^GTNACC
BstXI                          CCANNNNN^NTGG
BtgZI                          GCGATG (10/14)
BtrI                           CACGTC (-3/-3)
BtsI                           GCAGTG (2/0)
BtsIMutI                       CAGTG (2/0)
Cac8I                          GCN^NGC
CauII                          CC^SGG
CchII                          GGARGA (11/9)
CchIII                         CCCAAG (20/18)
CcrNAIII                       CGACCAG
CdiI                           CATCG (-1/-1)
Cdi630V                        CAAAAA
CdpI                           GCGGAG (20/18)
CfrI                           Y^GGCCR
Cfr10I                         R^CCGGY
Cgl13032I                      GGCGCA
Cgl13032II                     ACGABGG
CjeI                           (8/14) CCANNNNNNGT (15/9)
CjeFIII                        GCAAGG
CjeFV                          GGRCA
CjeNII                         GAGNNNNNGT
CjeNIII                        GKAAYG (19/17)
CjePI                          (7/13) CCANNNNNNNTC (14/8)
CjeP659IV                      CACNNNNNNNGAA
CjuI                           CAYNNNNNRTG
CjuII                          CAYNNNNNCTC
ClaI                           AT^CGAT
CspCI                          (11/13) CAANNNNNGTGG (12/10)
CstMI                          AAGGAG (20/18)
CviJI                          RG^CY
CviRI                          TG^CA
DdeI                           C^TNAG
DpnI                           GA^TC
DraII                          RG^GNCCY
DraIII                         CACNNN^GTG
DraRI                          CAAGNAC (20/18)
DrdI                           GACNNNN^NNGTC
DrdII                          GAACCA
DsaI                           C^CRYGG
Eam1105I                       GACNNN^NNGTC
EciI                           GGCGGA (11/9)
Eco31I                         GGTCTC (1/5)
Eco47III                       AGC^GCT
Eco57I                         CTGAAG (16/14)
Eco57MI                        CTGRAG (16/14)
EcoNI                          CCTNN^NNNAGG
EcoRI                          G^AATTC
EcoRII                         ^CCWGG
EcoRV                          GAT^ATC
EsaSSI                         GACCAC
EspI                           GC^TNAGC
Esp3I                          CGTCTC (1/5)
FaiI                           YA^TR
FalI                           (8/13) AAGNNNNNCTT (13/8)
FauI                           CCCGC (4/6)
FinI                           GGGAC
FnuDII                         CG^CG
Fnu4HI                         GC^NGC
FokI                           GGATG (9/13)
FseI                           GGCCGG^CC
FspAI                          RTGC^GCAY
FspEI                          CC (12/16)
GauT27I                        CGCGCAGG
GdiII                          CGGCCR (-5/-1)
GlaI                           GC^GC
GsuI                           CTGGAG (16/14)
HaeI                           WGG^CCW
HaeII                          RGCGC^Y
HaeIII                         GG^CC
HaeIV                          (7/13) GAYNNNNNRTC (14/9)
HgaI                           GACGC (5/10)
HgiAI                          GWGCW^C
HgiCI                          G^GYRCC
HgiEII                         ACCNNNNNNGGT
HgiJII                         GRGCY^C
HhaI                           GCG^C
Hin4I                          (8/13) GAYNNNNNVTC (13/8)
Hin4II                         CCTTC (6/5)
HindII                         GTY^RAC
HindIII                        A^AGCTT
HinfI                          G^ANTC
HpaI                           GTT^AAC
HpaII                          C^CGG
HphI                           GGTGA (8/7)
Hpy99I                         CGWCG^
Hpy99XIII                      GCCTA
Hpy99XIV                       GGWTAA
Hpy99XIV-mut1                  GGWCNA
Hpy99XXII                      TCANNNNNNTRG
Hpy178III                      TC^NNGA
Hpy188I                        TCN^GA
HpyAXIV                        GCGTA
HpyAXVI-mut2                   CRTCNA
HpyAXVI-mut1                   CRTTAA
Jma19592I                      GTATNAC
KpnI                           GGTAC^C
KroI                           G^CCGGC
Ksp632I                        CTCTTC (1/4)
LpnPI                          CCDG (10/14)
MaeI                           C^TAG
MaeII                          A^CGT
MaeIII                         ^GTNAC
MaqI                           CRTTGAC (21/19)
MauBI                          CG^CGCGCG
MboI                           ^GATC
MboII                          GAAGA (8/7)
McrI                           CGRY^CG
MfeI                           C^AATTG
MjaIV                          GTNNAC
MkaDII                         GAGAYGT
MluI                           A^CGCGT
MmeI                           TCCRAC (20/18)
MnlI                           CCTC (7/6)
MseI                           T^TAA
MslI                           CAYNN^NNRTG
MspJI                          CNNR (9/13)
MstI                           TGC^GCA
MwoI                           GCNNNNN^NNGC
NaeI                           GCC^GGC
NarI                           GG^CGCC
NcoI                           C^CATGG
NdeI                           CA^TATG
NgoAVIII                       (12/14) GACNNNNNTGA (13/11)
NhaXI                          CAAGRAG
NheI                           G^CTAGC
NlaIII                         CATG^
NlaIV                          GGN^NCC
NlaCI                          CATCAC (19/17)
NmeAIII                        GCCGAG (21/19)
NotI                           GC^GGCCGC
NruI                           TCG^CGA
NspI                           RCATG^Y
NspBII                         CMG^CKG
OliI                           CACNN^NNGTG
PacI                           TTAAT^TAA
PasI                           CC^CWGGG
PcsI                           WCGNNNN^NNNCGW
PenI                           GCAGT
Pfl1108I                       TCGTAG
PflMI                          CCANNNN^NTGG
PfoI                           T^CCNGGA
PleI                           GAGTC (4/5)
PmaCI                          CAC^GTG
PmeI                           GTTT^AAAC
PpiI                           (7/12) GAACNNNNNCTC (13/8)
PpuMI                          RG^GWCCY
PshAI                          GACNN^NNGTC
PsiI                           TTA^TAA
PspOMII                        CGCCCAR (20/18)
PspPRI                         CCYCAG (15/13)
PspXI                          VC^TCGAGB
PsrI                           (7/12) GAACNNNNNNTAC (12/7)
PstI                           CTGCA^G
PvuI                           CGAT^CG
PvuII                          CAG^CTG
RceI                           CATCGAC (20/18)
RdeGBI                         CCGCAG
RdeGBII                        ACCCAG (20/18)
RdeGBIII                       (9/11) TGRYCA (11/9)
RflFIII                        CGCCAG
RlaI                           VCW
RleAI                          CCCACA (12/9)
RpaI                           GTYGGAG (11/9)
RpaBI                          CCCGCAG (20/18)
RpaB5I                         CGRGGAC (20/18)
RpaTI                          GRTGGAG
RsaI                           GT^AC
RsrII                          CG^GWCCG
SacI                           GAGCT^C
SacII                          CCGC^GG
SalI                           G^TCGAC
SanDI                          GG^GWCCC
SapI                           GCTCTTC (1/4)
SauI                           CC^TNAGG
ScaI                           AGT^ACT
ScrFI                          CC^NGG
SdeAI                          CAGRAG (21/19)
SdeOSI                         (11/13) GACNNNNRTGA (12/10)
SduI                           GDGCH^C
SecI                           C^CNNGG
Sen1736II                      GATCAG
SenTFV                         GATCAG
SetI                           ASST^
SexAI                          A^CCWGGT
SfaNI                          GCATC (5/9)
SfeI                           C^TRYAG
SfiI                           GGCCNNNN^NGGCC
SgeI                           CNNGNNNNNNNNN^
SgfI                           GCGAT^CGC
SgrAI                          CR^CCGGYG
SgrDI                          CG^TCGACG
SgrTI                          CCDS (10/14)
SimI                           GGGTC (-3/0)
SmaI                           CCC^GGG
SmlI                           C^TYRAG
SnaI                           GTATAC
SnaBI                          TAC^GTA
Sno506I                        GGCCGAG
SpeI                           A^CTAGT
SphI                           GCATG^C
SplI                           C^GTACG
SpoDI                          GCGGRAG
SrfI                           GCCC^GGGC
Sse232I                        CG^CCGGCG
Sse8387I                       CCTGCA^GG
Sse8647I                       AG^GWCCT
SspI                           AAT^ATT
SstE37I                        CGAAGAC (20/18)
Sth132I                        CCCG (4/8)
StuI                           AGG^CCT
StyI                           C^CWWGG
SwaI                           ATTT^AAAT
TaqI                           T^CGA
TaqII                          GACCGA (11/9)
TatI                           W^GTACW
TauI                           GCSG^C
TfiI                           G^AWTC
TseI                           G^CWGC
TsoI                           TARCCA (11/9)
Tsp45I                         ^GTSAC
Tsp4CI                         ACN^GT
TspDTI                         ATGAA (11/9)
TspEI                          ^AATT
TspGWI                         ACGGA (11/9)
TspRI                          CASTGNN^
TssI                           GAGNNNCTC
TstI                           (8/13) CACNNNNNNTCC (12/7)
TsuI                           GCGAC
Tth111I                        GACN^NNGTC
Tth111II                       CAARCA (11/9)
UbaF9I                         TACNNNNNRTGT
UbaF11I                        TCGTA
UbaF12I                        CTACNNNGTC
UbaF13I                        GAGNNNNNNCTGG
UbaF14I                        CCANNNNNTCG
UbaPI                          CGAACG
VspI                           AT^TAAT
WviI                           CACRAG (21/19)
XbaI                           T^CTAGA
XcmI                           CCANNNNN^NNNNTGG
XhoI                           C^TCGAG
XhoII                          R^GATCY
XmaIII                         C^GGCCG
XmnI                           GAANN^NNTTC



	    TYPE I ENZYMES
	    ---------------

CfrAI                          GCANNNNNNNNGTGG
CjeFII                         CAAYNNNNNNACT
CjeNIV                         TAAYNNNNNTGC
Eco377I                        GGANNNNNNNNATGC
Eco394I                        GACNNNNNRTAAY
Eco585I                        GCCNNNNNNTGCG
Eco646I                        CCANNNNNNNCTTC
Eco777I                        GGANNNNNNTATC
Eco826I                        GCANNNNNNCTGA
Eco851I                        GTCANNNNNNTGAY
Eco912I                        CACNNNNNTGGC
EcoAI                          GAGNNNNNNNGTCA
EcoBI                          TGANNNNNNNNTGCT
EcoDI                          TTANNNNNNNGTCY
EcoDR2                         TCANNNNNNGTCG
EcoDR3                         TCANNNNNNNATCG
EcoDXXI                        TCANNNNNNNRTTC
EcoEI                          GAGNNNNNNNATGC
EcoKI                          AACNNNNNNGTGC
EcoR124I                       GAANNNNNNRTCG
EcoR124II                      GAANNNNNNNRTCG
EcoRD2                         GAANNNNNNRTTC
EcoRD3                         GAANNNNNNNRTTC
EcoprrI                        CCANNNNNNNRTGC
Hpy99XVI                       RTAYNNNNNRTAY
HpyAXVIII                      GGANNAG
KpnAI                          GAANNNNNNTGCC
KpnBI                          CAAANNNNNNRTCA
LlaBIII                        TNAGCC
LlaGI                          CTNGAYG
NgoAV                          GCANNNNNNNNTGC
StyLTIII                       GAGNNNNNNRTAYG
StySBLI                        CGANNNNNNTACC
StySEAI                        ACANNNNNNTYCA
StySGI                         TAANNNNNNRTCG
StySJI                         GAGNNNNNNGTRC
StySKI                         CGATNNNNNNNGTTA
StySPI                         AACNNNNNNGTRC
StySQI                         AACNNNNNNRTAYG



	    TYPE III ENZYMES
	    ---------------

BceJI                          CACAG
BceSI                          CGAAG (27/29)
Btr192II                       ACATC
EcoGVIII                       ACCACC
EcoPI                          AGACC (25/27)
EcoP15I                        CAGCAG (25/27)
GmeII                          TCCAGG
HinfIII                        CGAAT
MmyCI                          TGAG
NgoAX                          CCACC
PstII                          CTGATG (25/27)
StyLTI                         CAGAG

