EMBOSS: wordfinder

| wordfinder

## Wiki

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## Function

Match large sequences against one or more other sequences

## Description

This is a rough and ready local alignment program for large sequences. The reason it is rough and ready is that wordmatch is used to find all the word matches between the first sequence and another sequence. Then by calculating the highest score for a diagonal we can then use this as the centre point for a Smith-Waterman type calculation of a width given by the user. So a narrow diagonal Smith-Waterman is calculated hence the results will be rough but due to the space saving much larger sequences can be aligned.

## Usage

Here is a sample session with wordfinder

    % wordfinder tembl:j01636 @eclac.list -word 50
    Match large sequences against one or more other sequences
    Gap opening penalty [30.0]:
    Gap extension penalty [1.5]: 3.0
    Output alignment [j01636.wordfinder]:

Go to the input files for this example
Go to the output files for this example

## Command line arguments

    Match large sequences against one or more other sequences
    Version: EMBOSS:6.5.6.0

       Standard (Mandatory) qualifiers:
      [-asequence]         seqset     Sequence set filename and optional format,
                                      or reference (input USA)
      [-bsequence]         seqall     Sequence(s) filename and optional format, or
                                      reference (input USA)
       -gapopen            float      [10.0 for any sequence type] Gap opening
                                      penalty (Number from 0.000 to 1000.000)
       -gapextend          float      [0.5 for any sequence type] Gap extension
                                      penalty (Number from 0.000 to 10.000)
      [-outfile]           align      [*.wordfinder] Output alignment file name
                                      (default -aformat simple)

       Additional (Optional) qualifiers:
       -datafile           matrixf    [EBLOSUM62 for protein, EDNAFULL for DNA]
                                      This is the scoring matrix file used when
                                      comparing sequences. By default it is the
                                      file 'EBLOSUM62' (for proteins) or the file
                                      'EDNAFULL' (for nucleic sequences). These
                                      files are found in the 'data' directory of
                                      the EMBOSS installation.
       -width              integer    [16] Alignment width (Integer 1 or more)
       -wordlen            integer    [6] Word length for initial matching
                                      (Integer 3 or more)
       -limitmatch         integer    [0] Maximum match score (zero for no limit)
                                      (Integer 0 or more)
       -limitalign         integer    [0] Maximum alignment length (zero for no
                                      limit) (Integer 0 or more)
       -lowmatch           integer    [0] Minimum match score (zero for no limit)
                                      (Integer 0 or more)
       -lowalign           integer    [0] Minimum alignment length (zero for no
                                      limit) (Integer 0 or more)
       -errfile            outfile    [wordfinder.error] Error file to be written
                                      to

       Advanced (Unprompted) qualifiers: (none)
       Associated qualifiers:

       "-asequence" associated qualifiers
       -sbegin1            integer    Start of each sequence to be used
       -send1              integer    End of each sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -scircular1         boolean    Sequence is circular
       -sformat1           string     Input sequence format
       -iquery1            string     Input query fields or ID list
       -ioffset1           integer    Input start position offset
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-bsequence" associated qualifiers
       -sbegin2            integer    Start of each sequence to be used
       -send2              integer    End of each sequence to be used
       -sreverse2          boolean    Reverse (if DNA)
       -sask2              boolean    Ask for begin/end/reverse
       -snucleotide2       boolean    Sequence is nucleotide
       -sprotein2          boolean    Sequence is protein
       -slower2            boolean    Make lower case
       -supper2            boolean    Make upper case
       -scircular2         boolean    Sequence is circular
       -sformat2           string     Input sequence format
       -iquery2            string     Input query fields or ID list
       -ioffset2           integer    Input start position offset
       -sdbname2           string     Database name
       -sid2               string     Entryname
       -ufo2               string     UFO features
       -fformat2           string     Features format
       -fopenfile2         string     Features file name

       "-outfile" associated qualifiers
       -aformat3           string     Alignment format
       -aextension3        string     File name extension
       -adirectory3        string     Output directory
       -aname3             string     Base file name
       -awidth3            integer    Alignment width
       -aaccshow3          boolean    Show accession number in the header
       -adesshow3          boolean    Show description in the header
       -ausashow3          boolean    Show the full USA in the alignment
       -aglobal3           boolean    Show the full sequence in alignment

       "-errfile" associated qualifiers
       -odirectory         string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-asequence] (Parameter 1) | seqset | Sequence set filename and optional format, or reference (input USA) | Readable set of sequences | Required
[-bsequence] (Parameter 2) | seqall | Sequence(s) filename and optional format, or reference (input USA) | Readable sequence(s) | Required
-gapopen | float | Gap opening penalty | Number from 0.000 to 1000.000 | 10.0 for any sequence type
-gapextend | float | Gap extension penalty | Number from 0.000 to 10.000 | 0.5 for any sequence type
[-outfile] (Parameter 3) | align | Output alignment file name | (default -aformat simple) | <*>.wordfinder
Additional (Optional) qualifiers
-datafile | matrixf | This is the scoring matrix file used when comparing sequences. By default it is the file 'EBLOSUM62' (for proteins) or the file 'EDNAFULL' (for nucleic sequences). These files are found in the 'data' directory of the EMBOSS installation. | Comparison matrix file in EMBOSS data path | EBLOSUM62 for protein EDNAFULL for DNA
-width | integer | Alignment width | Integer 1 or more | 16
-wordlen | integer | Word length for initial matching | Integer 3 or more | 6
-limitmatch | integer | Maximum match score (zero for no limit) | Integer 0 or more | 0
-limitalign | integer | Maximum alignment length (zero for no limit) | Integer 0 or more | 0
-lowmatch | integer | Minimum match score (zero for no limit) | Integer 0 or more | 0
-lowalign | integer | Minimum alignment length (zero for no limit) | Integer 0 or more | 0
-errfile | outfile | Error file to be written to | Output file | wordfinder.error
Advanced (Unprompted) qualifiers
(none)
Associated qualifiers
"-asequence" associated seqset qualifiers
-sbegin1 -sbegin_asequence | integer | Start of each sequence to be used | Any integer value | 0
-send1 -send_asequence | integer | End of each sequence to be used | Any integer value | 0
-sreverse1 -sreverse_asequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_asequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_asequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_asequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_asequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_asequence | boolean | Make upper case | Boolean value Yes/No | N
-scircular1 -scircular_asequence | boolean | Sequence is circular | Boolean value Yes/No | N
-sformat1 -sformat_asequence | string | Input sequence format | Any string |
-iquery1 -iquery_asequence | string | Input query fields or ID list | Any string |
-ioffset1 -ioffset_asequence | integer | Input start position offset | Any integer value | 0
-sdbname1 -sdbname_asequence | string | Database name | Any string |
-sid1 -sid_asequence | string | Entryname | Any string |
-ufo1 -ufo_asequence | string | UFO features | Any string |
-fformat1 -fformat_asequence | string | Features format | Any string |
-fopenfile1 -fopenfile_asequence | string | Features file name | Any string |
"-bsequence" associated seqall qualifiers
-sbegin2 -sbegin_bsequence | integer | Start of each sequence to be used | Any integer value | 0
-send2 -send_bsequence | integer | End of each sequence to be used | Any integer value | 0
-sreverse2 -sreverse_bsequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask2 -sask_bsequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide2 -snucleotide_bsequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein2 -sprotein_bsequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower2 -slower_bsequence | boolean | Make lower case | Boolean value Yes/No | N
-supper2 -supper_bsequence | boolean | Make upper case | Boolean value Yes/No | N
-scircular2 -scircular_bsequence | boolean | Sequence is circular | Boolean value Yes/No | N
-sformat2 -sformat_bsequence | string | Input sequence format | Any string |
-iquery2 -iquery_bsequence | string | Input query fields or ID list | Any string |
-ioffset2 -ioffset_bsequence | integer | Input start position offset | Any integer value | 0
-sdbname2 -sdbname_bsequence | string | Database name | Any string |
-sid2 -sid_bsequence | string | Entryname | Any string |
-ufo2 -ufo_bsequence | string | UFO features | Any string |
-fformat2 -fformat_bsequence | string | Features format | Any string |
-fopenfile2 -fopenfile_bsequence | string | Features file name | Any string |
"-outfile" associated align qualifiers
-aformat3 -aformat_outfile | string | Alignment format | Any string | simple
-aextension3 -aextension_outfile | string | File name extension | Any string |
-adirectory3 -adirectory_outfile | string | Output directory | Any string |
-aname3 -aname_outfile | string | Base file name | Any string |
-awidth3 -awidth_outfile | integer | Alignment width | Any integer value | 0
-aaccshow3 -aaccshow_outfile | boolean | Show accession number in the header | Boolean value Yes/No | N
-adesshow3 -adesshow_outfile | boolean | Show description in the header | Boolean value Yes/No | N
-ausashow3 -ausashow_outfile | boolean | Show the full USA in the alignment | Boolean value Yes/No | N
-aglobal3 -aglobal_outfile | boolean | Show the full sequence in alignment | Boolean value Yes/No | N
"-errfile" associated outfile qualifiers
-odirectory | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

wordfinder reads two sets of nucleotide or protein sequences.

The input is a standard EMBOSS sequence query (also known as a 'USA').

Major sequence database sources defined as standard in EMBOSS installations include srs:embl, srs:uniprot and ensembl

Data can also be read from sequence output in any supported format written by an EMBOSS or third-party application.

The input format can be specified by using the command-line qualifier `-sformat xxx`, where 'xxx' is replaced by the name of the required format. The available format names are: gff (gff3), gff2, embl (em), genbank (gb, refseq), ddbj, refseqp, pir (nbrf), swissprot (swiss, sw), dasgff and debug.

See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further information on sequence formats.

### Input files for usage example

'tembl:j01636' is a sequence entry in the example nucleic acid database 'tembl'

### File: eclac.list

    #Formerly ECLAC
    tembl:J01636

    #Formerly ECLACA
    tembl:X51872

    #Formerly ECLACI
    tembl:V00294

    #Formerly ECLACY
    tembl:V00295

    #Formerly ECLACZ
    tembl:V00296

### Database entry: tembl:j01636

    ID   J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP.
    XX
    AC   J01636; J01637; K01483; K01793;
    XX
    DT   30-NOV-1990 (Rel. 26, Created)
    DT   09-SEP-2004 (Rel. 81, Last updated, Version 8)
    XX
    DE   E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
    XX
    KW   acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
    KW   lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene;
    KW   lacZ gene; mutagenesis; palindrome; promoter region;
    KW   thiogalactoside acetyltransferase.
    XX
    OS   Escherichia coli
    OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
    OC   Enterobacteriaceae; Escherichia.
    XX
    RN   [1]
    RP   1243-1266
    RX   DOI; 10.1073/pnas.70.12.3581.
    RX   PUBMED; 4587255.
    RA   Gilbert W., Maxam A.;
    RT   "The nucleotide sequence of the lac operator";
    RL   Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973).
    XX
    RN   [2]
    RP   1246-1308
    RX   DOI; 10.1073/pnas.70.12.3585.
    RX   PUBMED; 4587256.
    RA   Maizels N.M.;
    RT   "The nucleotide sequence of the lactose messenger ribonucleic acid
    RT   transcribed from the UV5 promoter mutant of Escherichia coli";
    RL   Proc. Natl. Acad. Sci. U.S.A. 70(12):3585-3589(1973).
    XX
    RN   [3]
    RX   PUBMED; 4598642.
    RA   Gilbert W., Maizels N., Maxam A.;
    RT   "Sequences of controlling regions of the lactose operon";
    RL   Cold Spring Harb. Symp. Quant. Biol. 38:845-855(1974).
    XX
    RN   [4]
    RA   Gilbert W., Gralla J., Majors A.J., Maxam A.;
    RT   "Lactose operator sequences and the action of lac repressor";
    RL   (in) Sund H., Blauer G. (Eds.);
    RL   PROTEIN-LIGAND INTERACTIONS:193-207;
    RL   Walter de Gruyter, New York (1975)
    XX
    RN   [5]
    RP   1146-1282

      [Part of this file has been deleted for brevity]

         cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg      4560
         ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg      4620
         ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct      4680
         ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg      4740
         gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga      4800
         aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct      4860
         gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct      4920
         ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg      4980
         cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta      5040
         agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg      5100
         gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct      5160
         ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac      5220
         ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg      5280
         ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct      5340
         cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg      5400
         tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc      5460
         tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca      5520
         atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg      5580
         gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc      5640
         gtcaggtgaa tgaagtcgct taagcaatca atgtcggatg cggcgcgacg cttatccgac      5700
         caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga ataagagcag      5760
         gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt gggaaaacgt      5820
         taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa agcctgatta      5880
         aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc tatttctctt      5940
         acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta accattgtcg      6000
         atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt actctttccg      6060
         ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac tcttttccga      6120
         taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca ggcgtcacca      6180
         tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt ccaccaaacg      6240
         tcgtggcggc tggcgttcct tgtcgggtta ttcgcgaaat aaacgaccgg gataagcact      6300
         attatttcaa agattataaa gttgaatcgt cagtttaaat tataaaaatt gcctgatacg      6360
         ctgcgcttat caggcctaca agttcagcga tctacattag ccgcatccgg catgaacaaa      6420
         gcgcaggaac aagcgtcgca tcatgcctct ttgacccaca gctgcggaaa acgtactggt      6480
         gcaaaacgca gggttatgat catcagccca acgacgcaca gcgcatgaaa tgcccagtcc      6540
         atcaggtaat tgccgctgat actacgcagc acgccagaaa accacggggc aagcccggcg      6600
         atgataaaac cgattccctg cataaacgcc accagcttgc cagcaatagc cggttgcaca      6660
         gagtgatcga gcgccagcag caaacagagc ggaaacgcgc cgcccagacc taacccacac      6720
         accatcgccc acaataccgg caattgcatc ggcagccaga taaagccgca gaaccccacc      6780
         agttgtaaca ccagcgccag cattaacagt ttgcgccgat cctgatggcg agccatagca      6840
         ggcatcagca aagctcctgc ggcttgccca agcgtcatca atgccagtaa ggaaccgctg      6900
         tactgcgcgc tggcaccaat ctcaatatag aaagcgggta accaggcaat caggctggcg      6960
         taaccgccgt taatcagacc gaagtaaaca cccagcgtcc acgcgcgggg agtgaatacc      7020
         acgcgaaccg gagtggttgt tgtcttgtgg gaagaggcga cctcgcgggc gctttgccac      7080
         caccaggcaa agagcgcaac aacggcaggc agcgccacca ggcgagtgtt tgataccagg      7140
         tttcgctatg ttgaactaac cagggcgtta tggcggcacc aagcccaccg ccgcccatca      7200
         gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc cgtttaatca      7260
         ccgaagcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag      7320
         cagcagcgca ctttgcgggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag      7380
         actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc      7440
         cagcccgccc atggtaacca ccggcagagc ggtcgac                               7477
    //

## Output file format

The output is a standard EMBOSS alignment file.

The results can be output in one of several styles by using the command-line qualifier `-aformat xxx`, where 'xxx' is replaced by the name of the required format. Some of the alignment formats can cope with an unlimited number of sequences, while others are only for pairs of sequences.

The available multiple alignment format names are: multiple, simple, fasta, msf, clustal, mega, meganon, nexus,, nexusnon, phylip, phylipnon, selex, treecon, tcoffee, debug, srs.

The available pairwise alignment format names are: pair, markx0, markx1, markx2, markx3, markx10, match, sam, bam, score, srspair

See: http://emboss.sf.net/docs/themes/AlignFormats.html for further information on alignment formats.

Bt default the output is in 'simple' format.

### Output files for usage example

### File: wordfinder.error

    Target 1 J01636 matches 1
    Target 2 X51872 matches 1
    Target 3 V00294 matches 1
    Target 4 V00295 matches 1
    Target 5 V00296 matches 1

### File: j01636.wordfinder

    ########################################
    # Program: wordfinder
    # Rundate: Sun 15 Jul 2012 12:00:00
    # Commandline: wordfinder
    #    [-asequence] tembl:j01636
    #    [-bsequence] @../../data/eclac.list
    #    -wordlen 50
    #    -gapextend 3.0
    # Align_format: simple
    # Report_file: j01636.wordfinder
    ########################################

    #=======================================
    #
    # Aligned_sequences: 2
    # 1: J01636
    # 2: J01636
    # Matrix: EDNAFULL
    # Gap_penalty: 30.0
    # Extend_penalty: 3.0
    #
    # Length: 7477
    # Identity:    7477/7477 (100.0%)
    # Similarity:  7477/7477 (100.0%)
    # Gaps:           0/7477 ( 0.0%)
    # Score: 37385.0
    #
    # Wordscore:7477
    # Alignlength:7477
    #
    #=======================================

    J01636             1 gacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgccc     50
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    J01636             1 gacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgccc     50

    J01636            51 ggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacg    100
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    J01636            51 ggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacg    100

    J01636           101 atgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg    150
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    J01636           101 atgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg    150

    J01636           151 aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggc    200
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    J01636           151 aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggc    200

    J01636           201 gatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgg    250
                         ||||||||||||||||||||||||||||||||||||||||||||||||||

      [Part of this file has been deleted for brevity]

    J01636          3787 tgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttattt   3836
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2501 tgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttattt   2550

    J01636          3837 atcagccggaaaacctaccggattgatggtagtggtcaaatggcgattac   3886
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2551 atcagccggaaaacctaccggattgatggtagtggtcaaatggcgattac   2600

    J01636          3887 cgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcc   3936
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2601 cgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcc   2650

    J01636          3937 tgaactgccagctggcgcaggtagcagagcgggtaaactggctcggatta   3986
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2651 tgaactgccagctggcgcaggtagcagagcgggtaaactggctcggatta   2700

    J01636          3987 gggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccg   4036
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2701 gggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccg   2750

    J01636          4037 ctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcg   4086
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2751 ctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcg   2800

    J01636          4087 aaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccag   4136
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2801 aaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccag   2850

    J01636          4137 tggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaact   4186
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2851 tggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaact   2900

    J01636          4187 gatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggc   4236
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2901 gatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggc   2950

    J01636          4237 tgaatatcgacggtttccatatggggattggtggcgacgactcctggagc   4286
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          2951 tgaatatcgacggtttccatatggggattggtggcgacgactcctggagc   3000

    J01636          4287 ccgtcagtatcggcggaattccagctgagcgccggtcgctaccattacca   4336
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    V00296          3001 ccgtcagtatcggcggaattccagctgagcgccggtcgctaccattacca   3050

    J01636          4337 gttggtctggtgtcaaaaataataataa   4364
                         ||||||||||||||||||||||||||||
    V00296          3051 gttggtctggtgtcaaaaataataataa   3078

    #---------------------------------------
    #---------------------------------------

The file 'wordfinder.error' will contain any errors that occured during the program. This may be that wordmatch could not find any matches hence no suitable start point is found for the smith-waterman calculation.

## Data files

For protein sequences EBLOSUM62 is used for the substitution matrix. For nucleotide sequence, EDNAMAT is used. Others can be specified.

EMBOSS data files are distributed with the application and stored in the standard EMBOSS data directory, which is defined by the EMBOSS environment variable EMBOSS_DATA.

To see the available EMBOSS data files, run:

    % embossdata -showall

To fetch one of the data files (for example 'Exxx.dat') into your current directory for you to inspect or modify, run:

    % embossdata -fetch -file Exxx.dat

Users can provide their own data files in their own directories. Project specific files can be put in the current directory, or for tidier directory listings in a subdirectory called ".embossdata". Files for all EMBOSS runs can be put in the user's home directory, or again in a subdirectory called ".embossdata".

The directories are searched in the following order:

- . (your current directory)
- .embossdata (under your current directory)
- ~/ (your home directory)
- ~/.embossdata

## Notes

The time this program takes to do an alignment depends very much on the word size. For short sequences a short word size (e.g. 4) can make it take a very long time. Large word sizes (e.g. 30) for sequences that are very similar give a very quick result. The default of 16 should give reasonable fast alignments.

Because it does a Smith & Waterman alignment (albeit in a narrow region around the diagonal shown to be the 'best' by a word match), this program can use huge amounts of memory if the sequences are large.

Because the alignment is made within a narrow area each side of the 'best' diagonal, if there are sufficient indels between the two sequences, then the path of the Smith & Waterman alignment can wander outside of this area. Making the width larger can avoid this problem, but you then use more memory.

The longer the sequences and the wider the specified alignment width, the more memory will be used.

If the program terminates due to lack of memory you can try the following:

Run the UNIX command 'limit' to see if your stack or memory usage have been limited and if so, run 'unlimit', (e.g.: '% unlimit stacksize').

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It always exits with a status of 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
matcher | Waterman-Eggert local alignment of two sequences
seqmatchall | All-against-all word comparison of a sequence set
supermatcher | Calculate approximate local pair-wise alignments of larger sequences
water | Smith-Waterman local alignment of sequences
wordmatch | Find regions of identity (exact matches) of two sequences

## Author(s)

Ian Longden formerly at:
Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.

## History

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None